FluoTag®-X2 anti-TagFPCat no:
€ 110.00 EUR
FluoTag®-X2 anti-TagFP is derived from an in-house developed single-domain antibody (sdAb) that recognizes fluorescent proteins deriving from Entacmaea quadricolor, such as mTagBFP, mTagRFP, mTagRFP657 and mKate with high affinity and specificity. For a complete specificity chart see the Product Specificity Chart below. Our FluoTag-X2 series features two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X2 series can thus improve the localization accuracy by 10-15 nm. Both features – enhanced brightness and precise fluorophore placement – render our FluoTag-X2 products superior tools for all microscopy techniques.
Specificity: Recognizes mTagBFP, mTagRFP and mKate and most common fluorescent proteins deriving from Entacmaea quadricolor. Does not cross-react with common GFP- or dsRed derivatives. For more detailed information see Product Reactivity Chart.
Produced in: E.coli
Concentration: 2.5 µM protein, 5 µM fluorophore
Recommended dilution for IF/ ICC: 1:500