NanoTag's FluoTag®-X2 species-specific anti-immunoglobulin secondary tools are alpaca single-domain antibodies, also referred to as Nanobodies® (trademark of Ablynx, Inc). With only ~15kD, they are 10-times smaller than conventional IgGs. Since Nanobodies are monovalent, they can directly be incubated with primary antibodies without forming non-functional clusters. Besides saving time on immunofluorescence stainings and Western blotting, the FluoTags® enable researchers to perform innovative applications with a precision and quality unreachable using conventional secondary antibodies.
The FluoTags for Western Blotting are conjugated to Licor dyes to enable quantitative imaging with high precision.
FluoTag®-X2 anti-Mouse is a species-specific FluoTag® directed against the IgG1 subtype from Mouse. In our FluoTag-X2 series, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Mouse antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.
Antigen: Mouse IgG1 Specificity: Recognizes native Mouse IgG1. It does not cross-react with Mouse IgG2a/b/c or IgG3. Does not cross-react with Guinea Pig, Rat, Rabbit, Chicken, Donkey, Goat, Pig, Horse, Cow or Human IgGs. Produced in:E.coli Concentration: 5 µM protein, 10 µM fluorophore; 0.2 mg/mL sdAB anti-IgG1 conjugated to HRP Recommended dilution for WB: 1:500 (LiCor), 1:2000 (HRP conjugate)