FluoTag-X2 anti-Rabbit for WesternBlotting

FluoTag-X2 anti-Rabbit for WesternBlotting

Cat no: N1002-Li680-S

Regular price € 150.00 EUR Sale

NanoTag's FluoTag®-X2 species-specific anti-immunoglobulin secondary tools are alpaca single-domain antibodies, also referred to as Nanobodies® (trademark of Ablynx, Inc). With only ~15kD, they are 10-times smaller than conventional IgGs. Since Nanobodies are monovalent, they can directly be incubated with primary antibodies without forming non-functional clusters. Besides saving time on immunofluorescence stainings and Western blotting, the FluoTags® enable researchers to perform innovative applications with a precision and quality unreachable using conventional secondary antibodies.

 

The FluoTags for Western Blotting are conjugated to Licor dyes to enable quantitative imaging with high precision. 

FluoTag®-X2 anti-Rabbit is an isotype- and species-specific FluoTag® directed against immunoglobulins from Rabbit. In our FluoTag-X2 series, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets two fluorophores to your primary Rabbit antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there are no batch-to-batch variations.

Antigen: Rabbit IgG
Specificity: Recognizes native Rabbit IgG. It does not cross-react with Mouse, Guinea Pig, Chicken, Rat, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
Produced in: E.coli
Concentration: 5 µM protein, 10 µM fluorophore
Recommended dilution for WB: 1:500