FluoTag®-X2 anti-SYT1

FluoTag®-X2 anti-SYT1

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FluoTag®-X2 anti-Synaptotagmin 1 (SYT1) is an alpaca single-domain antibody (sdAb) that recognizes specifically the cytoplasmic C-terminal domain of murine Synaptotagmin 1 with high affinity and specificity. Our X2 series features two site-specifically coupled fluorophores per sdAb. This reagent brings precisely two fluorophores to each Synaptotagmin 1 molecule, which results in an enhanced signal that increases linearly with the number of targets. The FluoTag®-X2 anti-Synaptotagmin 1 is validated for immunofluorescence and immunohistochemistry applications. In contrast to conventional antibodies, our FluoTags are 10x smaller and have an enhanced penetration in tissue. They are also monovalent, which avoids probe-induced artifacts like clustering of target molecules and they do not need to be revealed using secondary antibodies. Our FluoTag®-X2 anti-Synaptotagmin 1 is therefore ideally suited for various microscopy applications.

Specificity: Recognizes the cytosolic domain of mouse Synaptotagmin 1. Also recognizes rat Synaptotagmin 1 (other species not tested)
Concentration: 2.5 µM protein and 5 µM fluorophore
Recommended dilution for IF/ ICC: 1:500